Accutesttest HbsgAg (50 Tests)

Principle:
AccuTest HBsAg is a qualitative test based on immunochromatography sandwich principle. The test card includes a combination of
monoclonal anti-body gold conjugate (colloidal gold) and polyclonal solid phase antibodies which selectively binds Hepatitis B
surface antigen with high degree of sensitivity.
The HBsAg test is a one-step immunochromatographic assay based on the antigen capture, or Sandwich principle. The method
uses monoclonal antibodies conjugated to colloidal gold and polyclonal antibodies immobilized on a nitrocellulose strip in a thin
line. The test sample is introduced into well and flows laterally through an absorbent pad where it mixes with the signal reagent. If
sample contains HBsAg, the colloidal gold-antibody (mouse) conjugate binds to the antigen, forming an antigen-antibody-colloidal
gold complex. The complexes then migrate through the nitrocellulose strip by capillary action, which are stopped by an immobilized antibody zone forming a purple band. The formation of the first purple band (T zone) is indicative of hepatitis positive. To serve as a procedural control, an additional line of antibody antimouse IgG has been immobilized on the card. If the test is performed correctly, this will result in the formation of purple and upon contact with the conjugate as a control line.